ABSTRACT
PURPOSE: Peripheral blood stem cells (PBSC) can be mobilized by use of G-CSF alone from normal bone marrow. In this study, feasibility of mobilization, collection, and hematologic recovery after transplantion were evaluated. METHPDS: From normal undamaged bone marrow of normal PBSC donors and patients with high risk brain tumor who had no experience of chemotherapy or radiotherapy, PBSC was mobilized by use of G-CSF alone. Ten ug/kg/day of G-CSF was injected subcutaneously and leukaphereses were done on the fourth and fifth day of G-CSF injection. Nucleated cells (NC), mononuclear cells (MNC), CD34+ cells and colony forming cells (CFC) were counted. Hematologic recovery was evaluated in 4 autologous transplantations and 6 allogeneic transplantations, 4 of which were done after T cell depletion. RESULTS: Twenty four leukaphereses were done in 6 normal donors and 6 patients with high risk brain tumor. Median 603.3 (342.6~834.5) mL/kg of blood was processed for median 447 (392~549) minutes. Collected cells were as follows; NC: 11.88 (3.11~25.89)x108/kg of donor, MNC: 8.66 (2.61~12.84)x10(8)/kg of donor, CD34+ cells: 7.05 (2.95~11.73)x10(6)/kg of donor, CFC: 25.38 (3.62~35.27)x10(5)/kg of donor, respectively. In allogeneic transplantation, time to reach absolute neutrophil count (ANC)> 500/uL, 1,000/ uL and platelet count> 20,000/uL, 50,000/uL were 10 (9~15) days, 11 (9~16) days, 11 (8~30) days, and 32 (19~156) days, respectively. In autologous transplantation, time to reach ANC> 500/uL, 1,000/uL and platelet count> 20,000/uL, 50,000/uL were 9.5 (9~11) days, 10.0 (9~11) days, 10.5 (9~13) days, and 14.5 (13~17) days, respectively. CONCLUSION: Sufficient number of undamaged PBSC was collected from normal bone marrow by use of G-CSF alone. Hematologic recovery after transplantaion was more rapid than allogeneic bone marrow transplantation or autologous PBSCT which was done with cells collected after chemotherapy and/or radiotherapy.